YANG Hua    School of Environment

Abstract: Grassland in northern China is a very important ecological barrier in China. It plays an important role in greening ecological environment, exporting favorable resources and ecological evolution sites. Due to global climate change and human factors, grassland in northem China is continuously deteriorated, causing many ecological and environmental problems. Nitrogen cycling and nitrogen fertility retention in grassland soil are the main limiting factors to play the important function of grassland ecosystem, while nitrogen cycle and nitrogen fertility retention capacity are closely related to the characteristics of soil organic nitrogen components and the enzyme activity related to mineralization turnover. The application of nitrogen fertilizer to grassland in China will soon become a common grassland management measure. However, the application of nitrogen fertilizer, especially the improper application of nitrogen fertilizer, will cause serious soil nitrogen loss, resulting in a series of environmental pollution such as low nitrogen utilization rate. groundwater pollution, eutrophication of the surrounding surface water and emission of N20 greenhouse gases. In recent years, urease inhibitor NBPT and nitrification inhibitor DMPP have attracted many scholars' attention because they can effectively improve nitrogen use efficiency and slow down nitrogen loss. However, the effects of nitrogen and inhibitors on the soil nitrogen content, availability and the relationship with the activity of nitrogen mineralization turnover enzymes are important but lack. The topic of nole. This paper, based on the Eergu'Na forest grassland ecosystem research station of the Shenyang Institute of Applied Ecology of the Chinese Academy of Sciences, uses a dual factor randomized block design to set up no fertilizer, urea (Urea), organic manure - cow dung (OM), 1/2 urea +1/2 organic manure (1/2 (U +O)) and CK and urease inhibitors (NBPT:N- butyl thiosinphosphates). Acyl three amine), nitrification inhibitor (DMPP: two methyl pyrazole phosphate), urease inhibitor + nitrification inhibitor combination (N+D) were interactively added. The change characteristics of nitrogen components were studied by acid hydrolysis distillation method in the soil of 0-10cm soil layer, and the nitrogen related mineralization tumover was studied by spectrophotometer. The changes in enzyme activity (protease, amidase, urease and N-acetyl beta -D- glucosidase), and the relationship between nitrogen component and related mineralizing enzyme activity by redundancy analysis, in order to reveal the response characteristics of soil nitrogen component and nitrogen mineralization.

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