Zichao Wang, Shengyu Yuan, Youtao Song, Congting Sun, Naishun Bu, 

                                                                              College of Environment Science, Liaoning University

                                                                                                                 Zhiwei Deng, 

                                                                               College of Environment and Chemical Engineering,

                                                                                                               Yuejing Wang, 

                                                                      Shandong Provincial Yantai Eco-Environment Monitoring Center

                                                                                                               Shilong Deng,

                                                                              College of Forestry, Northeast Forestry University

                                                                                                                Xinruo Wang, 

                                                   Liaoning Provincial Ecology and Environment Protection Science and Technology Center

Graphical Abstract

Abstract

The responses of nitrification and denitrification to the divalent zinc (Zn(II)) and tetracycline (TC) co-selective pressure were evaluated in a sequencing batch reactor (SBR). The removal rates of organics and nitrogen, nitrifying and denitrifying enzymatic activity, and microbial diversity and richness at the Zn(II) and TC co-selective pressure were higher than those at the alone Zn(II) selective pressure, while were lower than those at the individual TC selective pressure. The Zn(II) and TC co-selective pressure induced the TC resistance genes abundance increase and the Zn(II) resistance genes levels decrease, and enhanced bacterial enzymatic modification resistance to TC and bacterial outer membrane resistance to Zn(II). The network analysis showed that the genera Nitrospira and Nitrosomonas of nitrifiers and the genera Ferruginibacter, Dechloromonas, Acidovorax, Rhodobacter, Thauera, Cloacibacterium, Zoogloea and Flavobacterium of denitrifiers were the potential hosts of antibiotics resistance genes (ARGs) and/or heavy metals resistance genes (HMRGs).

Read the article here:       59-Evaluating responses of nitrification and denitrification to the co-selective pressure of divalent zinc and tetracycline based on resistance genes changes.pdf