Ying Xia, Xiaoyu Liu, Dan Wang, Zechen Wang, Qiang Liu, Haibo Yu, Youtao Song

School of Environment, Liaoning University

Mingyan Zhang

 Liaoning Center of Disease Prevention and Control

Abstract

Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO^- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe (Rh-ClO) based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO detection in water samples and HeLa cells. Upon addition of ClO^-, color changes of solution from colorless to pink were immediately visible to the nakedeyes, meanwhile, brilliant red fluorescence was observed under excited at UV light (365 nm). Rh-ClO displayed high selectivity and sensitivity for ClO^-, and the detection limit was 7mmol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO^- within 10 s, Rh-ClO and its test paper could successfully detect ClO in water. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO^- in water samples and in mitochondria of living cells.

Read the article here:   2-A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples.pdf