Zechen Wang , Qinghao Zhang , Junwen Liu , Ran Sui, Yahui Li, Yue Li, Haibo Yu, Kui Jing 

School of Environment, Liaoning University

Xinfu Zhang, Yi Xiao

Dalian University of Technology

Mingyan Zhang

Liaoning Center of Disease Prevention and Control

Abstract

A novel six-membered rhodamine-based fluorescent probe (6G-ClO) was developed from 2-formyl rhodamine (6G-CHO) and used for hypochlorite detection in water and HUVEC cells. Different from planar penta cycle of rhodamine spirolactam, there was a twist six-membered spirocyclic hydrazone in 6G-ClO optimized by Gaussian software at DFT/B3LYP/6-31G(d) level. The high selectivity, high sensitivity and fast response of 6G-ClO towards ClO^-  would be attributed to the twist six-membered spiro-cycle. Test-strip prepared with 6G-ClO was successfully used to semi-quantitatively indicate the concentration of ClO^- in water. 6G-ClO can also quantitatively detect the concentration of ClO^- in tap water and swimming pool water. The detection limit of 6G-ClO was as low as 12 nM. The co-localization staining of HUVEC cells further verified that 6G-ClO could specifically accumulate in lysosomes and capture exogenous/endogenous ClO^- in living lysosomes. 6G-ClO would be a practical probe for real-time monitoring of ClO^- in the biological and real water samples.

Read the article here:   9-A twist six-membered rhodamine-based fluorescent probe for hypochlorite detection in water and lysosomes of living cells.pdf